Interaction of lipogenic substrates in porcine adipose tissue in vitro

1. Porcine adipose tissue was incubated with radiolabeled glucose, acetate or lactate. Saturation curves indicated that lactate greater than glucose greater than acetate in providing two-carbon units for fatty-acid synthesis. 2. Competition between individual substrates indicated that lactate was the best lipogenic substrate. 3. Incubation of all three substrates at concentrations observable in serum indicated that at 5.56 mM, glucose was the preferred lipogenic substrate in the presence of 0.1 mM acetate and 1.0 mM lactate. 4. At elevated concentrations (18.52 mM glucose, 1.0 mM acetate and 10.0 mM lactate), acetate and lactate were preferred to glucose as lipogenic substrates.