Related Experiment Video
Updated: Jun 17, 2026
Generation and Single-Cell Transcriptomic Analysis of Hepatocellular Carcinoma Organoids following Drug Treatment
Published on: May 26, 2026
Proteome profiling of arsenic trioxide-treated human hepatic cancer cells
Dong Ryeol Yoo1, Seon Ah Chong, Myeong Jin Nam
1Gachon University of Medicine and Science, 534-2 Yeonsu-dong, Yeonsu-gu, Incheon, Republic of Korea.
Background:
Arsenic trioxide (As(2)O(3)), a major compound in traditional Chinese medicine, is known to be an effective anticancer agent in acute promyelocytic leukemia (APL). The effects of As(2)O(3) on human hepatocellular carcinoma (HCC) SK-Hep-1 cells were studied employing proteomics-based methodologies.
Materials And Methods:
Using 1-dimensional electrophoresis (1DE) and liquid chromatography electrospray ionization quadruple time-of-flight analysis, the whole proteomes of the control and As(2)O(3)-treated cells were profiled.
Results:
In all, 207 and 62 proteins, which were specifically found in control and As(2)O(3)-treated cells, respectively, were classified with their biological processes by gene ontology (GO) annotation. The GO data indicated that 16 proteins were closely associated with apoptotic mechanisms. As(2)O(3)-induced DNA damage and oxidative stress that accompanied apoptosis in SK-Hep-1 cells were observed using comet assay and 5-and-6-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate fluorescence microscopy, respectively.
Conclusion:
The anticancer activities of As(2)O(3) may be mediated by DNA damage- and reactive oxygen species-induced apoptotic mechanisms which involve the proteins identified in this study.
