[Change in the hybridization characteristics of rapidly-labelled ribonucleic acids during tumor "progression"]

The hybridization properties of in vivo rapidly labeled with 14C-orotate both nuclear and mitochondrial ribonucleic acids from the MD hepatoma were investigated. During tumour progression the repression of nuclear genome found at its early stages (5th to 6th passages) is replaced by the increase of hybridizability of nuclear DNA with a population of 14C-RNA's as well as by the appearance of new classes of pulse labeled RNA's. In other words, at late stages of tumour progression (60th passage) there occur a de-repression of nuclear genome. The hybridizability of mitochondrial RNA with nuclear DNA remains almost the same at different tumour progression stages. The results obtained are discussed in the light of literature data available.