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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Related Experiment Video

Updated: Jun 17, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
06:53

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

[Basic methods for extraction and molecular analysis of micromycetic DNA].

K V Bogdanov, S M Ignat'eva

    Klinicheskaia Laboratornaia Diagnostika
    |December 17, 2009
    PubMed
    Summary

    This study presents an improved DNA extraction method using glass microbeads for fungi, ensuring high yield and integrity. This method enables accurate identification and quantification of fungal pathogens in clinical samples, crucial for immunocompromised patients.

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    Last Updated: Jun 17, 2026

    Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
    06:53

    Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

    Published on: May 5, 2023

    Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
    12:02

    Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

    Published on: May 2, 2018

    Whole-Genome Deoxyribonucleic Acid Extraction from Mycobacterium Species via the Cetyltrimethylammonium Bromide Technique
    06:46

    Whole-Genome Deoxyribonucleic Acid Extraction from Mycobacterium Species via the Cetyltrimethylammonium Bromide Technique

    Published on: December 12, 2025

    Area of Science:

    • Molecular Biology
    • Mycology
    • Clinical Diagnostics

    Context:

    • Fungal infections pose significant risks, especially to immunocompromised individuals.
    • Accurate identification and quantification of fungal pathogens are critical for effective treatment.
    • Traditional DNA extraction methods can struggle with rigid fungal cell walls, leading to low yield and DNA degradation.

    Purpose:

    • To develop and optimize a DNA extraction method for fungal nucleic acids from clinical samples.
    • To ensure high yield and minimize degradation of DNA from various microorganisms, particularly mycelial fungi.
    • To validate the suitability of the extracted DNA for molecular diagnostic techniques.

    Summary:

    • A modified DNA extraction protocol utilizing glass microbeads effectively lyses rigid fungal cell walls, yielding high-quality, intact DNA from fungi and their spores.
    • The extracted DNA is suitable for various molecular analyses, including qualitative/quantitative polymerase chain reaction (PCR), real-time PCR, and sequencing for ribosomal gene polymorphism.
    • Combining quantitative PCR and sequencing allows for the simultaneous determination of gene target copy numbers for up to nine different micromycetes in clinical specimens.

    Impact:

    • This method enhances the accuracy and efficiency of diagnosing fungal infections in clinical settings.
    • It is particularly beneficial for patients with compromised immune systems, such as organ transplant recipients.
    • Improved fungal DNA extraction facilitates precise diagnosis and targeted treatment strategies, improving patient outcomes.