Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fibril-associated Collagen01:11

Fibril-associated Collagen

Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Type IV Collagen of Basal Lamina01:05

Type IV Collagen of Basal Lamina

Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen  forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can exist in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In situ</i>rheological monitoring of diffusion-controlled hydrogel crosslinking for embedded 3D bioprinting.

Biofabrication·2026
Same author

Antifoam hindrance of air release in lubricating oils.

Journal of colloid and interface science·2026
Same author

Cell-free systems for expression of transmembrane protein.

Progress in molecular biology and translational science·2026
Same author

Cell-free systems for biotransformation.

Progress in molecular biology and translational science·2026
Same author

Macrophage-targeted PEGylated liposomes ameliorate experimental autoimmune encephalomyelitis.

Frontiers in immunology·2026
Same author

Intraventricular iron causes severe hydrocephalus - a model of severe neonatal hydrocephalus.

Fluids and barriers of the CNS·2025
Same journal

Controlled Secondary Growth of CAU-1-NH<sub>2</sub> Membranes with Improved CO<sub>2</sub> Separation Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Facile Fabrication and Stable Mechanism of a Microscale Heavy Calcium Carbonate Suspension.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Polycationic Biocidal Coatings: The Mechanism of Their Interaction with Cells.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Atomic-Scale Displacement in Ordered SmMnO<sub>3</sub> Nanoislands.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Vacancy Defect Modulated Interfacial Thermal Transport and Phonon Localization in AlGaN/GaN Heterojunctions.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Immobilization of Ytterbium via Polyphenol Chemistry on Implant Materials for Enhanced Cytocompatibility and Antibacterial Properties.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
10:24

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

Interfacial flow processing of collagen.

An J J Goffin1, Jayakumar Rajadas, Gerald G Fuller

  • 1Department of Chemical Engineering, Stanford University, Stanford, California 94305, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel method for creating aligned collagen fiber substrates. These ordered collagen scaffolds promote cell alignment in culture, advancing tissue engineering and implant coating technologies.

More Related Videos

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

Related Experiment Videos

Last Updated: Jun 17, 2026

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
10:24

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Cell alignment on substrates is crucial for tissue regeneration and understanding cellular behavior.
  • Current methods for creating ordered collagen substrates are often complex or use non-biocompatible materials.

Purpose of the Study:

  • To develop a simple and effective method for producing well-controlled, oriented collagen substrates.
  • To investigate the influence of interfacial conditions on collagen fiber formation and orientation.
  • To evaluate the response of cultured cells to these oriented collagen substrates.

Main Methods:

  • Collagen solutions were spread at the air-saline interface to form fibers.
  • High-speed compression was used to orient the collagen fibers.
  • Langmuir-Blodgett transfer was employed to deposit oriented fibers onto solid substrates.
  • Interfacial techniques studied collagen behavior influenced by salt concentration and temperature.
  • Primary human fibroblasts and adipose-derived stem cells were cultured on the substrates.

Main Results:

  • Collagen fiber formation and orientation were successfully achieved using the described interfacial method.
  • Collagen behavior was found to be dependent on subphase salt concentration and temperature.
  • Both fibroblasts and stem cells cultured on the oriented substrates aligned with the collagen fibers.
  • The substrates demonstrated favorable cell adhesion and alignment without additives.

Conclusions:

  • A straightforward technique for generating oriented collagen substrates was established.
  • These substrates provide a promising platform for cell culture research and tissue engineering scaffolds.
  • The method offers a biocompatible approach for improving cell adhesion on implant surfaces.