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Updated: Jun 17, 2026
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
Published on: September 13, 2016
Antiangiogenic response after 70% hepatectomy and its relationship with hepatic regeneration and angiogenesis in rats
Ahmet Bulent Dogrul1, Tamer Colakoglu, Kemal Kosemehmetoglu
1Department of General Surgery, Medical School, Hacettepe University, Ankara, Turkey. ahmetdogrul@yahoo.com
Background:
The aim of this study was to evaluate the antiangiogenic response and its relation to regeneration and angiogenesis after 70% hepatectomy in a rat model.
Methods:
Sixty-four Wistar albino rats were included in the study. Animals were allocated into 8 groups (n = 8). After a 70% hepatectomy, liver regeneration, angiogenesis, and antiangiogenic response were evaluated in the remnant liver on days 0, 1, 2, 3, 5, 7, 10, and 14. Regeneration and angiogenesis were determined with immunoreactivity to proliferating cell nuclear antigen and vascular endothelial growth factor. Antiangiogenic response was evaluated by detecting collagen 18 m RNA with reverse transcriptase polymerase chain reaction.
Results:
We showed that liver regeneration peaked at day 1, whereas angiogenesis in the periportal and perisinusoidal areas reached their peak values on days 3 and 7, respectively. Both regeneration and angiogenic activity around perisinusoidal hepatocytes returned to basal activity on the day 10. Antiangiogenic response first appeared on day 5, reached a peak on day 10, and returned to basal values on day 14.
Conclusion:
Collagen18 mRNA expression is present in the normal liver during the regenerative process. We suggest that the stimulus that causes the cessation of regeneration process may come from hepatocytes, and collagen 18 produced by hepatocytes may modulate this event by inhibiting the angiogenesis.
