Characterization of human serum albumin forms with pH. Fluorescence lifetime studies
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Updated: Jun 17, 2026

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging
Published on: March 27, 2020
Megdouda Amiri1, Kristina Jankeje, Jihad René Albani
1Laboratoire de Biophysique Moléculaire, Université des Sciences et Technologies de Lille, Bâtiment C6, 59655 Villeneuve d'Ascq Cédex, France.
Abstract:
Fluorescence lifetimes of human serum albumin (HSA) tryptophan 214 residue were measured in solution at different pH (from 2 to 12). The results indicate that tryptophan emission occurs with three lifetimes at all pH. However, lifetimes and pre-exponential values are dependent on the pH and thus on the protein form. Three different protein populations have been differentiated: one population for pH 2 and 3 (extended form), the second one from pH 4 to 9 containing HSA migrating (F), normal (N) and basic (B) forms. Another type of population is obvious for pH higher than 9, characterizing the aged (A) form of HSA.
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