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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jun 17, 2026

Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics
07:37

Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics

Published on: June 7, 2018

Fluorescence aptasensor based on competitive-binding for human neutrophil elastase detection.

Jing-Lin He1, Zai-Sheng Wu, Song-Bai Zhang

  • 1State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.

Talanta
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

We developed a novel fluorescence aptasensor for detecting human neutrophil elastase (HNE). This biosensor offers sensitive and linear detection of HNE in solution, with a low detection limit.

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Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
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Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Human neutrophil elastase (HNE) is a key protease implicated in various inflammatory diseases.
  • Sensitive and specific detection of HNE is crucial for disease diagnosis and monitoring.
  • Existing detection methods may have limitations in sensitivity, speed, or sample requirements.

Purpose of the Study:

  • To develop and validate the first fluorescence aptasensor for detecting human neutrophil elastase (HNE) in homogeneous solution.
  • To establish a sensitive and reliable method for HNE quantification.

Main Methods:

  • A fluorescence aptasensor was designed utilizing a molecular beacon (MB) and a short DNA scrambled sequence strand (SS).
  • The aptasensor relies on a competitive binding mechanism between the HNE aptamer, MB, and SS for HNE detection.
  • Fluorescence signals were measured to quantify HNE concentration.

Main Results:

  • The aptasensor demonstrated the ability to detect HNE in homogeneous solution.
  • A low detection limit of 47pM for HNE was achieved.
  • Linear response was observed in the concentration range of 0.34–68nM, with a sensitivity as low as 0.34nM.

Conclusions:

  • This study presents a novel and highly sensitive fluorescence aptasensor for HNE detection.
  • The developed biosensor shows promise for clinical applications requiring accurate HNE quantification.
  • The competitive binding strategy offers an effective platform for aptasensor development.