Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
Smooth Endoplasmic Reticulum01:21

Smooth Endoplasmic Reticulum

Smooth endoplasmic reticulum or smooth ER is a sub-organelle with specialized functions in animal cells and plant cells. It is often associated with the tubule morphology of the endoplasmic reticulum.
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
Myasthenia Gravis: Diagnostic Tests01:15

Myasthenia Gravis: Diagnostic Tests

Myasthenia gravis is an autoimmune condition affecting neuromuscular transmission, causing generalized weakness in skeletal muscles. Initial diagnoses rely on patients' signs, symptoms, and medical history. The challenge lies in distinguishing myasthenia from other muscular dystrophies. An important diagnostic feature is the significant improvement of symptoms after administering anticholinesterase inhibitors.
The edrophonium test is a diagnostic tool for myasthenia gravis. It involves...
Systematic Error: Methodological and Sampling Errors01:15

Systematic Error: Methodological and Sampling Errors

In the case of systematic errors, the sources can be identified, and the errors can be subsequently minimized by addressing these sources. According to the source, systematic errors can be divided into sampling, instrumental, methodological, and personal errors.
Sampling errors originate from improper sampling methods or the wrong sample population. These errors can be minimized by refining the sampling strategy. Defective instruments or faulty calibrations are the sources of instrumental...
Bioequivalence: Overview01:16

Bioequivalence: Overview

Pharmaceutical equivalents, by definition, are drug products with the same active ingredient in the same quantities, encapsulated in identical dosage forms, and intended for the same administration routes. These pharmaceutical equivalents are deemed bioequivalent if the bioavailability of the active entity in the drug preparations is similar. Moreover, pharmaceutical equivalents demonstrating bioequivalence are also regarded as therapeutically equivalent. This means that when used as directed,...
Titrimetric Methods: Types and Commonly Used Strategies01:08

Titrimetric Methods: Types and Commonly Used Strategies

In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel duodenal-release formulation of caraway oil and L-menthol is a safe, effective and well tolerated therapy for functional dyspepsia.

BMC gastroenterology·2022
Same author

Patient experience with NER1006 as a bowel preparation for colonoscopy: a prospective, multicenter US survey.

BMC gastroenterology·2021
Same author

High-quality colon cleansing and multiple neoplasia detection with 1L NER1006 versus mid-volume options: Post hoc analysis of phase 3 clinical trials.

Endoscopy international open·2020
Same author

Beyond the Label: Ensuring That Drug Benefits Outweigh Risks in the Food and Drug Administration's Risk Evaluation and Mitigation Strategy Program.

The American journal of gastroenterology·2019
Same author

Novel 1 L polyethylene glycol-based bowel preparation NER1006 for overall and right-sided colon cleansing: a randomized controlled phase 3 trial versus trisulfate.

Gastrointestinal endoscopy·2017
Same author

Nondestructive Determination of the Age of 20th-Century Oil-Binder Ink Prints Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR FT-IR): A Case Study with Postage Stamps from the Łódź Ghetto.

Applied spectroscopy·2016
Same journal

Single-cell mass spectrometry reveals metabolic divergence mediated by contact-dependent cell-cell interactions.

Talanta·2026
Same journal

Highly sensitive hydrogel surface-enhanced Raman scattering chips with multi-analyte detection ability.

Talanta·2026
Same journal

Quality by design-based RP-HPLC methods for pharmaceutical analytical estimation: A review.

Talanta·2026
Same journal

Development of Teduglutide purity certified reference material (GBW09342) through amino acid-based isotope dilution mass spectrometry and sulfur-based isotope dilution inductively coupled plasma mass spectrometry.

Talanta·2026
Same journal

Maximizing molecular coverage in ultrahigh-resolution orbitrap analysis of marine dissolved organic matter using two-stage design-of-experiments strategy.

Talanta·2026
Same journal

A new HPLC-ICP-MS method for gadolinium speciation in freshwaters using ion exchange chromatography and aqueous mobile phases.

Talanta·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Using Reference Reagents to Confirm Robustness of Cytokine Release Assays for the Prediction of Monoclonal Antibody Safety
06:37

Using Reference Reagents to Confirm Robustness of Cytokine Release Assays for the Prediction of Monoclonal Antibody Safety

Published on: September 15, 2023

The reference materials syndrome.

Michael S Epstein1

  • 1Department of Science, Mount Saint Mary's University, Emmitsburg, MD 21727, United States. epstein@msmary.edu

Talanta
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

Analysts reported elemental concentrations matching incorrect reference material values. This highlights potential issues in spectroscopic analysis and reference data accuracy.

More Related Videos

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure
08:40

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

Published on: March 21, 2017

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
07:49

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

Published on: August 16, 2017

Related Experiment Videos

Last Updated: Jun 17, 2026

Using Reference Reagents to Confirm Robustness of Cytokine Release Assays for the Prediction of Monoclonal Antibody Safety
06:37

Using Reference Reagents to Confirm Robustness of Cytokine Release Assays for the Prediction of Monoclonal Antibody Safety

Published on: September 15, 2023

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure
08:40

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

Published on: March 21, 2017

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
07:49

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

Published on: August 16, 2017

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Spectroscopic methods are crucial for determining elemental concentrations.
  • Reference materials are essential for validating analytical measurements.
  • Discrepancies between reported values and true values can arise from various sources.

Purpose of the Study:

  • To investigate instances where spectroscopic analysis results align with erroneous reference material data.
  • To identify potential causes for agreement with incorrect certified values in elemental analysis.
  • To assess the impact of inaccurate reference standards on analytical chemistry findings.

Main Methods:

  • Literature review of published spectroscopic analyses.
  • Case study analysis of specific examples.
  • Comparison of reported elemental concentrations with certified values from reference materials.

Main Results:

  • Identified cases where elemental concentrations from spectroscopic analysis matched subsequently invalidated reference values.
  • Highlighted the challenge of detecting errors when analytical results appear to validate incorrect standards.
  • Demonstrated the critical dependence of analytical accuracy on the reliability of reference materials.

Conclusions:

  • The study underscores the importance of rigorous validation of reference materials.
  • Findings suggest a need for improved methods to identify discrepancies in analytical data linked to flawed standards.
  • Emphasizes the potential for widespread analytical errors if reference material integrity is compromised.