Related Experiment Video

Updated: Jun 17, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

A soluble form of P74 can act as a per os infectivity factor to the Autographa californica multiple

Jeffrey M Slack1, Susan D Lawrence, Peter J Krell

  • 1Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.

The Journal of General Virology
|December 17, 2009
PubMed

Insights

The Autographa californica multicapsid nucleopolyhedrovirus P74 protein is crucial for insect baculovirus infection. A soluble form of P74, lacking its anchor, retains infectivity, suggesting it may interact with other viral proteins.

Area of Science:

  • Virology
  • Insect Pathology
  • Molecular Biology

Background:

  • Baculovirus occlusion-derived virions (ODVs) are essential for per os transmission in insect hosts.
  • The Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) P74 protein is an ODV envelope protein critical for infectivity.

Purpose of the Study:

  • To investigate the role of the P74 protein's domains in ODV per os infectivity.
  • To determine if a soluble form of P74 can restore infectivity to P74-null baculoviruses.

Main Methods:

  • Construction and characterization of N-terminal and C-terminal P74 truncation mutants.
  • Evaluation of mutant P74 proteins for their ability to rescue per os infectivity of a P74-null baculovirus (AcLP4).

Main Results:

  • A P74 truncation mutant lacking the C-terminal transmembrane anchor domain successfully rescued per os infectivity.
  • This indicates that the soluble portion of P74 is sufficient for its infectivity factor function.

Conclusions:

  • The C-terminal transmembrane anchor is not essential for P74's role in per os infectivity.
  • A soluble form of P74 can function independently, suggesting potential complex formation with other ODV envelope proteins.

More Related Videos

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter07:53

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter

Published on: April 17, 2026

Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Subviral Agents01:29

Subviral Agents

Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...