aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length
Jan M Bell1, John D Turnidge, Patiyan Andersson
1SA Pathology, Women's and Children's Hospital, 72 King William Rd., North Adelaide, SA 5006, Australia.
Antimicrobial Agents and Chemotherapy
|December 17, 2009
Abstract:
We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6')-Ib-cr that is capable of distinguishing between the two genetic aac(6')-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.
