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Related Experiment Video

Updated: Jun 17, 2026

Hydroquinone Based Synthesis of Gold Nanorods

Hydroquinone Based Synthesis of Gold Nanorods

Published on: August 10, 2016

Preparation of colloidal gold.

Constance Oliver¹

¹Department of Cell and Molecular Biology, Faculdade de Medicina de Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.

Methods in Molecular Biology (Clifton, N.J.)

|December 17, 2009

Summary

Colloidal gold probes are essential for electron microscopy immunocytochemistry. This study details simple, reproducible methods for laboratory production of gold sols with controlled particle sizes (2-40 nm) using tetrachloroauric acid and reducing agents.

Area of Science:

Materials Science
Biotechnology
Microscopy

Background:

Colloidal gold probes are vital tools in electron microscopy for immunocytochemical staining.
Their utility relies on precise control over particle size.

Purpose of the Study:

To detail laboratory methods for producing colloidal gold sols of various sizes.
To provide simple and reproducible protocols for gold sol preparation.

Main Methods:

Boiling tetrachloroauric acid solution with a reducing agent.
Varying the type and concentration of the reducing agent to control particle size.
Producing gold sols with particle sizes ranging from 2 to 40 nm.

Main Results:

Successful laboratory production of gold sols with controlled particle sizes.

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Demonstration of simple and highly reproducible batch-to-batch preparation.

Methods allow for customization of gold particle size based on experimental needs.

Conclusions:

The described methods offer a reliable way to synthesize colloidal gold probes for electron microscopy.
These protocols are accessible for laboratory use, ensuring consistent results.
Control over particle size is achievable through careful selection of reagents and concentrations.