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The clinical immunohistochemistry laboratory: regulations and troubleshooting guidelines

Patricia A Fetsch1, Andrea Abati

  • 1Laboratory of Pathology, Cytopathology Section, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
PubMed

Keywords:
immunohistochemistry lab standardsCLIA compliance guidelineslaboratory quality assurancediagnostic testing regulations

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Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...