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Bio-resolution of glycidyl (o, m, p)-methylphenyl ethers by Bacillus megaterium
Zhengfang Zhang1, Yanming Sheng, Keyi Jiang
1College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014 People's Republic of China.
Abstract:
A newly isolated Bacillus megaterium with epoxide hydrolase activity resolved racemic glycidyl (o, m, p)-methylphenyl ethers to give enantiopure epoxides in 84-99% enantiomeric excess and with 21-73 enantiomeric ratios. The (S)-enantiomer was obtained from rac-glycidyl (o or m)-methylphenyl ether while the (R)-epoxides was obtained from glycidyl p-methylphenyl ether. The observations are explained at the level by enzyme-substrate docking studies.
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