Method of simultaneous analysis of liposome components using HPTLC/FID

Area of Science:

• Analytical Chemistry
• Materials Science
• Pharmaceutical Sciences

Background:

• Liposomes are versatile lipid-based nanocarriers for bioactive molecules.
• Accurate characterization of lipid composition is crucial for liposome efficacy and safety.
• Existing methods for lipid quantification can be sample-intensive or lack comprehensiveness.

Purpose of the Study:

• To develop and validate a rapid, simultaneous analytical method for determining all lipid ingredients in liposome formulations.
• To enable precise quality control throughout the liposome preparation process.
• To minimize sample quantity required for comprehensive liposome characterization.

Main Methods:

• Development of a Thin Layer Chromatography with Flame Ionization Detector (TLC/FID) method.
• Separation of liposomal lipid components on silica gel-coated quartz rods.
• Detection and quantification using a hydrogen flame ionization detector generating a chromatogram.

Main Results:

• Successful simultaneous determination of multiple lipid ingredients in a single analysis.
• Demonstration of the method's applicability using minimal sample volumes.
• Generation of reproducible chromatograms for accurate lipid quantification.

Conclusions:

• The developed TLC/FID method offers an efficient and sensitive approach for comprehensive liposome characterization.
• This analytical technique is valuable for quality assessment of raw materials, process monitoring, and final product analysis in liposome preparation.
• The method supports robust quality control in pharmaceutical development and manufacturing of liposomal formulations.