Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Liposome-Encapsulated Antibiotics for the Therapy of Mycobacterial Infections.

Antibiotics (Basel, Switzerland)·2025

Same author

DNA Methylation in Periodontal Disease: A Focus on Folate, Folic Acid, Mitochondria, and Dietary Intervention.

International journal of molecular sciences·2025

Same author

Manganese Sulfanyl Porphyrazine-MWCNT Nanohybrid Electrode Material as a Catalyst for H<sub>2</sub>O<sub>2</sub> and Glucose Biosensors.

Sensors (Basel, Switzerland)·2024

Same author

Peptides Derived from the SARS-CoV-2 S2-Protein Heptad-Repeat-2 Inhibit Pseudoviral Fusion at Micromolar Concentrations: The Role of Palmitic Acid Conjugation.

International journal of molecular sciences·2024

Same author

'Science by consensus' impedes scientific creativity and progress: A simple alternative to funding biomedical research.

F1000Research·2024

Same author

Recent Strategies for Cancer Therapy: Polymer Nanoparticles Carrying Medicinally Important Phytochemicals and Their Cellular Targets.

Pharmaceutics·2023

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

Viscometric analysis of DNA-lipid complexes.

Sadao Hirota¹, Nejat Düzgüneş

¹Department of Material Science, Tokyo Denki University, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|December 17, 2009

Summary

Viscometric analysis characterizes DNA-cationic lipid complexes (lipoplexes) for gene therapy. This automated method quantifies colloidal properties like shape and purity, crucial for process and quality control.

Area of Science:

Biophysics
Materials Science

Background:

DNA-cationic lipid complexes, or lipoplexes, are vital gene carriers in molecular biology and gene therapy.
Their colloidal properties significantly influence transfection efficiency and product quality.
Characterizing these properties is essential for reliable lipoplex production and quality control.

Purpose of the Study:

To introduce an automated mini-capillary viscometer for characterizing lipoplex colloidal properties.
To establish a quantitative method for assessing lipoplex shape, purity, and colloidal stability.
To demonstrate the utility of viscometric analysis for process and quality control in lipoplex manufacturing.

Main Methods:

Development and application of an automated mini-capillary viscometer with a 0.5 ml sample volume.

More Related Videos

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Published on: February 10, 2022

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Related Experiment Videos

Last Updated: Jun 17, 2026

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Published on: February 10, 2022

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Viscometric analysis of plant DNA-distearyldimethylammonium chloride (DDAC) lipoplexes at a 1:4 charge ratio.

Calculation of the prolate/ellipsoidal axial ratio (a/b) as a shape parameter.

Main Results:

The automated viscometer enables precise characterization of lipoplex colloidal parameters.
An axial ratio (a/b) of 70 was determined for DNA-DDAC lipoplexes, serving as a superior shape parameter compared to others.
Viscometric analysis effectively quantifies lipoplex shape, purity, and colloidal stability.

Conclusions:

Automated viscometric analysis provides a robust method for characterizing lipoplex colloidal properties.
The determined shape parameter (a/b) is a reliable indicator for lipoplex quality control.
This technique is applicable to both lipoplexes and other bioactive polymer complexes.