Endomyocardial biopsy derived adherent proliferating cells - a potential cell source for cardiac tissue engineering
Marion Haag1, Sophie Van Linthout, Sebastian E A Schröder
1Tissue Engineering Laboratory, Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Tucholskystr. 2, 10117 Berlin, Germany. marion-renate.haag@charite.de
Journal of Cellular Biochemistry
|December 17, 2009
Summary
Researchers identified novel human cardiac-derived adherent proliferating (CAP) cells from heart biopsies. These cells are easily expanded and show potential for cardiac repair by promoting angiogenesis, not cardiomyocyte differentiation.
Area of Science:
- Cardiology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Heart disease remains a major cause of death.
- Cardiac stem cells (CSCs) show therapeutic promise but are difficult to isolate and expand.
- Clinical translation of CSCs is hindered by complex isolation and expansion protocols.
Purpose of the Study:
- To describe a novel population of human cardiac-derived adherent proliferating (CAP) cells.
- To assess the feasibility of isolating and expanding CAP cells from endomyocardial biopsies.
- To characterize the identity and functional potential of CAP cells for cardiac repair.
Main Methods:
- Isolation and expansion of CAP cells from small endomyocardial biopsies.
- Growth kinetics analysis to determine cell doubling time and expansion capacity.
- Gene expression profiling (microarray) and flow cytometry to identify cell markers.
- Multilinage differentiation assays to assess differentiation potential.
- Analysis of angiogenesis-related gene expression.
Main Results:
- CAP cells were reliably isolated and expanded, yielding 2.54 x 10^7 cells in passage 3.
- CAP cells lack hematopoietic (CD34, CD45) and mesenchymal stem cell (CD90) markers.
- CAP cells demonstrated no multilineage differentiation potential (adipocytes, osteoblasts, etc.) or cardiomyocyte differentiation.
- CAP cells express genes crucial for angiogenesis, including VEGF and angiopoietin-1.
- CAP cells possess a unique gene expression profile distinct from other cell types.
Conclusions:
- A unique cardiac tissue-derived cell type, CAP cells, has been identified.
- CAP cells are a potential source for cardiac repair due to efficient isolation and expansion.
- CAP cells primarily support cardiac repair through promoting angiogenesis rather than cardiomyocyte differentiation.
