Multianalyte electrochemical biosensor based on aptamer- and nanoparticle-integrated bio-barcode amplification.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Simultaneous detection of biomarkers like adenosine and thrombin is crucial for diagnosing various diseases.
• Existing detection methods often lack sensitivity or require complex procedures.
• Aptamer-based electrochemical sensors offer a promising alternative due to their specificity and potential for miniaturization.

Purpose of the Study:

• To develop a signal-on electrochemical sensing strategy for the simultaneous detection of adenosine and thrombin.
• To utilize aptamer switching structures for enhanced sensing performance.
• To achieve ultra-low detection limits for adenosine and thrombin in biological samples.

Main Methods:

• Fabrication of a "sandwich-type" sensing interface on an Au electrode using capture probes and bio-barcoded AuNPs.
• Employing aptamers that undergo structural changes upon target binding, leading to the release of bio-barcoded AuNPs.
• Quantification of released metal sulfide nanoparticles via anodic stripping voltammetry (ASV) for signal generation.

Main Results:

• Achieved simultaneous detection of adenosine and thrombin with a signal-on mechanism.
• Demonstrated ultra-low detection limits: 6.6 x 10⁻¹² M for adenosine and 1.0 x 10⁻¹² M for thrombin.
• The developed sensor exhibited excellent selectivity and detectability in biological samples.

Conclusions:

• The developed aptamer-based electrochemical sensor provides a sensitive and selective platform for simultaneous adenosine and thrombin detection.
• The combination of bio-barcoded AuNPs and ASV technology offers dual amplification for enhanced detection.
• This strategy holds significant potential for clinical diagnostics and biomedical research.