The isolated neonatal rat-cardiomyocyte used in an in vitro model for 'ischemia'. I. A morphological study

Cultured heart cells have been recently shown to be useful for analysing states of oxygen- and volume-restrictions, conditions that are known to simulate anoxia and ischemia at the cellular level. In the present study, we examined the ultrastructural damage caused to cultured neonatal rat heart cells when they were subjected to simulated ischemia by volume restricted anoxia ('ischemia') in an in vitro system. Both thin-sectioning and freeze-fracturing electron microscopy revealed a mitochondrial reorganization after 30 min of 'ischemia', whereas multilamellar structures could be detected inside the mitochondria after another 30 min. At this time-point, changes were also observed regarding the organization of the sarcolemma. In addition to a slight aggregation of the intramembranous particles (IMP's) we found an extensive extrusion of particle-free multilamellar membrane-structures, possibly due to a loss of the sarcolemma/cytoskeleton-interaction. These morphological changes are comparable to those previously observed in in vivo and Langendorff studies and the results of the present study therefore underline the usefulness of this recently introduced model for ischemia.