Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PIEZO1 variants that reduce open channel probability are associated with familial osteoarthritis.

The Journal of biological chemistry·2026
Same author

Astrocyte Enrichment of 3D Cortical Constructs Enhances Brain Repair.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Human-derived cardiac-neural microtissues reveal catecholaminergic polymorphic ventricular tachycardia is also a disease of the sympathetic neuron.

The Journal of physiology·2026
Same author

Targeted high-resolution sensing of volatile organic compounds by covalent nanopore detection.

Nature communications·2025
Same author

High-Resolution Patterned Delivery of Chemical Signals From 3D-Printed Picoliter Droplet Networks.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Rational Design Principles for <i>De Novo</i> α-Helical Peptide Barrels with Dynamic Conductive Channels.

Journal of the American Chemical Society·2025

Related Experiment Video

Updated: Jun 17, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Wrestling with native chemical ligation.

Hagan Bayley1, Stephen Cheley, Leon Harrington

  • 1Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom. hagan.bayley@chem.ox.ac.uk

ACS Chemical Biology
|December 19, 2009
PubMed
Summary

This study presents an improved semisynthesis method for potassium channels using native chemical ligation. This technique enables precise insertion of non-canonical amino acids, advancing membrane protein research and biotechnology applications.

More Related Videos

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
10:36

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Published on: July 27, 2016

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!
10:40

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

Published on: January 26, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
10:36

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Published on: July 27, 2016

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!
10:40

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

Published on: January 26, 2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Potassium channels are crucial membrane proteins regulating cellular functions.
  • Semisynthesis offers a route to engineer complex proteins.
  • Introducing non-canonical amino acids expands protein functionality.

Purpose of the Study:

  • To develop an improved method for potassium channel semisynthesis.
  • To enable the incorporation of non-canonical amino acids at any position within the polypeptide chain.
  • To enhance tools for membrane protein research and biotechnology.

Main Methods:

  • Utilized native chemical ligation for polypeptide chain construction.
  • Developed a semisynthetic strategy for potassium channel assembly.
  • Introduced short sequences with non-canonical amino acids.

Main Results:

  • Successfully achieved semisynthesis of a potassium channel.
  • Demonstrated the ability to incorporate non-canonical amino acids site-specifically.
  • Validated the method for introducing diverse amino acid sequences.

Conclusions:

  • The improved semisynthesis method enhances capabilities for studying membrane proteins.
  • This technology facilitates the engineering of potassium channels for biotechnological applications.
  • Advances in protein engineering open new avenues for fundamental research.