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Published on: January 7, 2019
[Patterns of Enterococcus faecalis in infected root canals: an in vitro study]
1Department of Stomatology, Peking University People's Hospital, Beijing 100044, China.
Summary
This study developed an in vitro root canal model to observe Enterococcus faecalis infection. The model revealed biofilm formation and dentinal tubule penetration by Enterococcus faecalis.
Area of Science:
- Microbiology
- Endodontics
- Dental Research
Background:
- Enterococcus faecalis is a common pathogen in persistent root canal infections.
- Understanding its behavior within the root canal system is crucial for effective treatment.
Purpose of the Study:
- To establish a reliable in vitro root canal model infected with Enterococcus faecalis.
- To investigate the morphology, distribution, and spatial arrangement of Enterococcus faecalis within infected root canals.
Main Methods:
- Human premolars were infected with Enterococcus faecalis in brain-heart infusion broth for 21 days.
- Roots were sectioned and analyzed using light microscopy (Brown & Brenn stain) and scanning electron microscopy (SEM).
Main Results:
- Enterococcus faecalis penetrated dentinal tubules up to 1000 µm.
- Dense bacterial aggregations (biofilms) were observed in the apical third, with planktonic bacteria in coronal and middle thirds.
- Control specimens showed no microbial presence.
Conclusions:
- Enterococcus faecalis forms biofilms on root canal walls and invades dentinal tubules.
- The established in vitro model is practical for evaluating antimicrobial strategies against intracanal bacteria.
