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Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

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Related Experiment Video

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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
08:12

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Published on: January 8, 2018

Functional proteomics of failed filtering blebs.

Takashi Kanamoto1, Nazariy Souchelnytskyi, Yoshiaki Kiuchi

  • 1Department of Ophthalmology and Visual Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. tkana@hiroshima-u.ac.jp

Molecular Vision
|December 19, 2009
PubMed
Summary

Researchers identified eight proteins in failed filtering blebs, including ribosomal S6 kinase 2 (RSK2). Decreased RSK2 expression in fibroblasts suggests its role in trabeculectomy wound healing.

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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
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Published on: February 24, 2021

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Proteomics

Background:

  • Trabeculectomy is a surgical procedure to treat glaucoma.
  • Filtering blebs are crucial for the success of trabeculectomy.
  • Understanding the molecular mechanisms of filtering bleb failure is essential for improving surgical outcomes.

Purpose of the Study:

  • To identify and characterize proteins associated with failed filtering blebs after trabeculectomy.
  • To determine the function of ribosomal S6 kinase 2 (RSK2) in the context of filtering bleb wound healing.

Main Methods:

  • Proteomic analysis of Tenon's tissues from failed filtering blebs and normal eyes.
  • Mass spectrometry for protein identification.
  • Immunohistochemistry to determine RSK2 location and expression.
  • RNA knockdown techniques to investigate RSK2 function in fibroblast cells stimulated with basic fibroblast growth factor (bFGF).

Main Results:

  • Eight proteins were differentially expressed in failed filtering blebs, including those involved in intracellular signaling.
  • RSK2 expression was decreased in failed blebs.
  • RSK2 knockdown inhibited bFGF-induced fibroblast proliferation.
  • RSK2 knockdown affected actin and mitogen-activated protein kinase (MAPK) expression and activity.

Conclusions:

  • Significant differences in protein expression exist between failed filtering blebs and normal Tenon's capsules.
  • RSK2 plays a role in fibroblast function, suggesting its involvement in the wound healing processes of filtering blebs.