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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

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A Microfluidic Chip for ICPMS Sample Introduction
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A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Fully automated liquid extraction-based surface sampling and ionization using a chip-based robotic nanoelectrospray

Vilmos Kertesz1, Gary J Van Berkel

  • 1Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA. kerteszv@ornl.gov

Journal of Mass Spectrometry : JMS
|December 19, 2009
PubMed
Summary
This summary is machine-generated.

A novel automated liquid extraction surface sampling device was developed for improved analysis. This system demonstrated reliable performance across various sample types, matching previous liquid extraction techniques.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Surface Science

Background:

  • Surface sampling is crucial for analyzing complex biological and chemical samples.
  • Existing methods often require manual operation or specialized equipment.
  • Automated, high-throughput surface sampling is needed for broader applications.

Purpose of the Study:

  • To report a fully automated liquid extraction-based surface sampling device.
  • To enable discrete spot sampling using a chip-based infusion nanoelectrospray ionization system.
  • To evaluate the device's performance on diverse sample surfaces.

Main Methods:

  • Utilized an Advion NanoMate chip-based infusion nanoelectrospray ionization system.
  • Employed advanced user interface software for robotic pipettor control.
  • Developed a liquid microjunction formation and withdrawal technique for surface sampling.
  • Tested on spotted sample arrays, dried blood spots, and thin tissue sections.

Main Results:

  • Successfully performed automated liquid extraction and surface sampling.
  • Enabled discrete spot analysis with precise control over microjunctions.
  • Generated qualitative and quantitative data consistent with established methods.
  • Demonstrated applicability to MALDI plates, dried blood spots, and tissue sections.

Conclusions:

  • The automated device offers a robust platform for surface sampling and analysis.
  • The system provides reliable performance across various analytically important surfaces.
  • This technology advances automated sample preparation for mass spectrometry.