Related Experiment Video
Updated: Jun 17, 2026

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
Published on: March 24, 2023
Differential phosphorylation of MAPK isoforms in keratinocyte cell line by contact allergens and irritant
P Mehrotra1, S Upadhyaya, V P Sinkar
1Environmental Safety Laboratory, Hindustan Lever Research Centre, Mumbai, India.
Abstract:
ABSTRACT Mitogen-activated protein kinase (MAPK) is an important mediator of cellular signaling pathways. In an attempt to investigate the role of these kinases in contact allergen/irritant-induced signaling cascades, we have utilized keratinocyte (A431 cell line) as antigen presenting cells (APCs). This group has previously reported the applicability of A431 as an APC. The role of these kinases in the elicitation pathways of allergic contact dermatitis was investigated using interleukin-12p40 (IL-12p40) as a marker. Inhibitors of the isoforms of MAPK reduced the allergen-induced IL-12 release in the A431 cell line. This result suggests a possible role of the isoforms of MAPK in the development of contact allergy.
Related Concept Videos
MAPK Signaling Cascades
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Allergic Reactions
Amplifying Signals via Enzymatic Cascade
Mitogens and the Cell Cycle
cAMP-dependent Protein Kinase Pathways
