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Application and detection of (14)c-hd in two mouse models
Thomas P Logan1, Michael Shutz, Susan M Schulz
1United States Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, Maryland, USA.
Toxicology Mechanisms and Methods
|December 22, 2009
Summary
This study compared sulfur mustard (HD) exposure in haired and hairless mice. Results show HD rapidly decreases in skin post-exposure, with similar HD quantities detected on both mouse models.
Area of Science:
- Toxicology
- Dermatology
- Animal Models
Background:
- Sulfur mustard (HD) is a chemical warfare agent.
- Animal models are crucial for studying HD exposure and protection.
- Previous studies showed differential protection efficacy between haired and hairless mouse models.
Purpose of the Study:
- To quantitatively compare sulfur mustard (HD) exposure and absorption kinetics in haired and hairless mouse models.
- To understand HD distribution within the skin of hairless mice.
- To establish a concentration/time value for HD vapor exposure in hairless mice.
Main Methods:
- Exposure of haired and hairless mice to radiolabeled sulfur mustard (HD) via topical application or vapor.
- Quantification of radiolabeled HD ((14)C) in skin samples at various time points post-exposure.
- Skin biopsy techniques to assess HD distribution.
- Determination of HD concentration/time values.
Main Results:
- (14)C content in skin decreased significantly within 30 minutes post-exposure across all models and exposure routes.
- HD application spread beyond the initial application site in hairless mice (ratio of 7.3).
- A concentration/time value of 6.3 µg/cm²/min was determined for saturated HD vapor exposure.
- Similar quantities of HD (0.4 mg) were detected on both mouse models.
Conclusions:
- Both haired and hairless mouse models exhibit rapid clearance of HD from the skin.
- The distribution of HD in the skin of hairless mice is wider than the initial application site.
- These findings provide a quantitative basis for using these mouse models in HD research.
