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A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
Published on: September 10, 2021
Adaptation of the highly productive T7 expression system to Streptomyces lividans
François-Xavier Lussier1, François Denis, François Shareck
1INRS-Institut Armand-Frappier, 531 Boul. des Prairies, Laval, Québec, Canada H7V 1B7. francois-xavier.lussier@iaf.inrs.ca
Applied and Environmental Microbiology
|December 22, 2009
Abstract:
Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.

