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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

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Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

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Bio-electrospraying embryonic stem cells: interrogating cellular viability and pluripotency.

Anil Abeyewickreme¹, Albert Kwok, Jean R McEwan

¹Molecular Immunology Unit, Institute of Child Health, University College London, Guilford Street, London, United Kingdom.

Integrative Biology : Quantitative Biosciences From Nano to Macro

|December 22, 2009

Summary

Bio-electrospraying, a novel cell engineering method, shows no harm to mouse embryonic stem cells. Treated cells maintain viability and pluripotency, expanding applications in regenerative medicine.

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Area of Science:

Biotechnology
Cell Biology
Regenerative Medicine

Background:

Bio-electrospraying is a direct electric field driven cell engineering process.
Previous studies confirmed no molecular harm to various cell types.
Embryonic stem cells are crucial for tissue engineering and regenerative medicine but haven't been studied with this method.

Purpose of the Study:

To assess the effects of bio-electrospraying on mouse embryonic stem cells.
To determine if the technique impacts cell viability and pluripotency.
To evaluate the potential of bio-electrospraying for embryonic stem cell applications.

Main Methods:

Mouse embryonic stem (ES) cells were subjected to bio-electrospraying.
Cell viability was assessed post-treatment.
Cell pluripotency was evaluated and compared to control groups.

Main Results:

Bio-electrospraying did not affect the viability of mouse embryonic stem cells.
Pluripotency of treated embryonic stem cells was indistinguishable from controls.
This study presents the first successful application of bio-electrospraying on embryonic stem cells.

Conclusions:

Bio-electrospraying is a safe and effective method for engineering embryonic stem cells.
The technique preserves essential stem cell characteristics, including viability and pluripotency.
This finding supports the expanded use of bio-electrospraying in regenerative medicine and tissue engineering.