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Updated: Jun 17, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Genome-wide transcriptome analysis of 150 cell samples.

Daniel Irimia¹, Michael Mindrinos², Aman Russom¹

¹BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Shriners Hospital for Children, and Harvard Medical School, Boston, MA 02114, USA.

Integrative Biology : Quantitative Biosciences From Nano to Macro

|December 22, 2009

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Researchers developed a new method for whole transcriptome analysis using minimal biological samples. This technique enables genome-wide gene expression profiling from as few as 150 cells, overcoming previous sample size limitations.

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Interrogating the human transcriptome genome-wide is challenging with limited biological samples.
Current microarray technologies require nanogram amounts of total RNA for mRNA transcription analysis.

Purpose of the Study:

To develop a method for global gene expression profiling from minimal biological samples.
To overcome the sample size limitation of existing transcriptome analysis technologies.

Main Methods:

Utilized microfluidic devices for total RNA purification from mammalian cells.
Employed ultra-sensitive whole transcriptome amplification techniques.
Analyzed samples as small as 150 cells (approximately 300 pg total RNA).

More Related Videos

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Main Results:

Preserved RNA integrity throughout the isolation process.
Achieved highly reproducible whole transcriptome analysis.
Demonstrated high correlation between repeated isolations from small cell numbers and bulk samples.
Successfully identified perturbed biological pathways consistent with bulk analysis.

Conclusions:

The developed method enables genome-wide transcriptome analysis from very small biological samples.
This approach overcomes the limitations of current technologies requiring larger sample quantities.
The technique is robust, reproducible, and capable of identifying biologically relevant gene expression changes.