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Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates

Young-Pil Kim1, Weston L Daniel, Zuyong Xia

  • 1Molecular Imaging Program at Stanford, Department of Radiology and Chemistry, Stanford University, Stanford, CA 94305-5484, USA.

Chemical Communications (Cambridge, England)
|December 22, 2009
PubMed

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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...