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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Updated: Jun 17, 2026

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Published on: January 7, 2016

CE-ESI-TOF/MS for human growth hormone analysis.

Aline Staub¹, Sylvain Giraud, Martial Saugy

¹School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.

Electrophoresis

|December 22, 2009

Summary

This study presents a fast and simple capillary electrophoresis-electrospray ionization-time of flight/mass spectrometry (CE-ESI-TOF/MS) method. It successfully distinguishes between endogenous and recombinant human growth hormone without prior sample preparation.

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Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

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Published on: January 7, 2016

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

Area of Science:

Analytical Chemistry
Biochemistry
Proteomics

Background:

Capillary Electrophoresis (CE) is a key technique for separating biomolecules like proteins.
Coupling CE with Time-of-Flight Mass Spectrometry (TOF/MS) enhances protein detection and identification capabilities.
Distinguishing between naturally occurring and engineered proteins is crucial in various biological and medical fields.

Purpose of the Study:

To develop an efficient, rapid, and simple CE-ESI-TOF/MS method for analyzing human growth hormone.
To analyze both endogenous human growth hormone and recombinant human growth hormone.
To achieve this analysis without the need for sample preparation.

Main Methods:

Capillary Electrophoresis coupled with Electrospray Ionization and Time-of-Flight Mass Spectrometry (CE-ESI-TOF/MS).
Optimization of operational parameters using experimental design methodologies.
Direct analysis of human growth hormone samples, bypassing traditional sample preparation steps.

Main Results:

An efficient, rapid, and simple CE-ESI-TOF/MS procedure was established.
The method demonstrated successful analysis of both endogenous and recombinant human growth hormone.
The optimized method effectively distinguished between the two forms of human growth hormone in unknown samples.

Conclusions:

The developed CE-ESI-TOF/MS method offers a powerful tool for protein analysis.
The technique provides a robust platform for differentiating protein variants, such as endogenous and recombinant human growth hormone.
This approach eliminates the need for sample preparation, streamlining the analytical workflow.