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Updated: Jun 17, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Nanoscale 3D tracking with conjugated polymer nanoparticles.

Jiangbo Yu1, Changfeng Wu, Sushant P Sahu

  • 1Department of Chemistry and Center for Optical Materials Science and Engineering Technologies, Clemson University, Clemson, South Carolina 29634, USA.

Journal of the American Chemical Society
|December 24, 2009
PubMed
Summary

Highly fluorescent conjugated polymer nanoparticles enable precise nanoscale tracking. These bright nanoparticles offer sub-nanometer accuracy for 2D and 3D biological imaging applications.

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Biophysics

Background:

  • Conjugated polymer nanoparticles offer unique optical properties.
  • High fluorescence brightness is crucial for sensitive nanoscale tracking.

Purpose of the Study:

  • Evaluate small (15 nm) conjugated polymer nanoparticles for nanoscale 2D and 3D tracking.
  • Determine the tracking precision and demonstrate applications in biological systems.

Main Methods:

  • Characterization of nanoparticle fluorescence and optical properties.
  • Analysis of particle trajectories for lateral and axial tracking uncertainty.
  • Demonstration of single-particle tracking in fixed cells.

Main Results:

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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

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Last Updated: Jun 17, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

  • Nanoparticles exhibit extraordinarily high fluorescent brightness.
  • Theoretical particle tracking uncertainty is less than 1 nm.
  • Experimental lateral tracking uncertainty of 1-2 nm was achieved.
  • 3D tracking was enabled using defocused imaging.

    • Conclusions:

    • Conjugated polymer nanoparticles are highly suitable for nanoscale 2D and 3D tracking.
    • Observed complex behaviors in cells suggest potential for studying binding/unbinding dynamics.
    • The technology offers a powerful tool for high-resolution biological imaging.