Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two-dimensional difference fluorescence gel electrophoresis to verify the scale-up of a non-affinity-based downstream process for isolation of a therapeutic recombinant antibody.

Electrophoresis·2010
Same author

Precise displacement measurement for a local surface.

Optics letters·2009
Same author

Diffractive barcode using grating-dot lines.

Optics letters·2008
Same author

Pattern composed of two-dimensional diffusion dots for showing dynamic images.

Applied optics·2007
Same author

Circadian expression of clock genes in purified hematopoietic stem cells is developmentally regulated in mouse bone marrow.

Experimental hematology·2006
Same author

Using random features of dot-matrix holograms for anticounterfeiting.

Applied optics·2006

Related Experiment Video

Updated: Jun 17, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Replacers of barcodes for small application areas by using grating dots to diffractively form bright points.

Sheng Lih Yeh1, Hung Jen Yang, Shyh Tsong Lin

  • 1Department of Mechanical Engineering, Lunghwa University of Science and Technology, 300, Section 1, Wanshou Road, Kueishan, Taoyuan County, Taiwan. slyeh@mail.lhu.edu.tw

Applied Optics
|December 24, 2009
PubMed
Summary

Pointcodes encode data using laser-light diffraction on grating patterns, enabling data management in very small areas where traditional barcodes fail. This novel optical method reconstructs data from diffracted light patterns.

More Related Videos

Fabrication of High Contrast Gratings for the Spectrum Splitting Dispersive Element in a Concentrated Photovoltaic System
12:08

Fabrication of High Contrast Gratings for the Spectrum Splitting Dispersive Element in a Concentrated Photovoltaic System

Published on: July 18, 2015

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Related Experiment Videos

Last Updated: Jun 17, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Fabrication of High Contrast Gratings for the Spectrum Splitting Dispersive Element in a Concentrated Photovoltaic System
12:08

Fabrication of High Contrast Gratings for the Spectrum Splitting Dispersive Element in a Concentrated Photovoltaic System

Published on: July 18, 2015

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Area of Science:

  • Optics and Data Storage
  • Diffractive Optics
  • Nanotechnology

Background:

  • Traditional barcodes are limited by size and cannot be applied to micro-scale applications.
  • Existing data management solutions lack efficiency for miniaturized devices.

Purpose of the Study:

  • To introduce pointcodes as a novel data encoding and decoding method for small areas.
  • To demonstrate the feasibility of using diffractive reconstruction for data retrieval.

Main Methods:

  • Encoding data into pointcode patterns using grating dots with varying pitches and orientations.
  • Utilizing laser illumination to diffractively reconstruct a pointcode image from the pattern.
  • Decoding data based on the positions and sizes of bright points in the reconstructed image.

Main Results:

  • Successfully reconstructed pointcode images from small pointcode patterns.
  • Demonstrated that pointcode patterns can encode numerical data using bright point configurations.
  • Achieved data diffraction and reconstruction under specific illuminating conditions.

Conclusions:

  • Pointcodes offer a viable solution for data management in micro-scale applications.
  • The diffractive reconstruction of pointcode images allows for high-density data storage and retrieval.
  • Small pointcode patterns are sufficient for effective data encoding and decoding.