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[RAPD analysis and construction of specific DNA probes between Pinellia ternata and Typhonium flagelliforme]
Guo Chen1, Quan Bai, Chao-li Wang
1Institute of Immunology & Molecular Biology, Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China.
Objective:
To establish a molecular marking method to identify Pinellia ternata and Typhonium flagelliforme.
Methods:
Twenty-two random oligonucleotide primers were used in RAPD analysis on the genomic DNA of two types of Pinellia ternata in Sichuan and two types of Typhonium flagelliforme in Guangxi. The special fragments were sequenced, marked as probes and then conducted Southern blot.
Results:
A great deal of special bands was found between Pinellia ternata and Typhonium flagelliforme. A Pinellia ternata specific molecule was screened.
Conclusion:
RAPD analysis and specific DNA probes show potential value in the identification of Pinellia ternata and Typhonium flagelliforme.
