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Related Experiment Video

Updated: Jun 17, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Rationally designed aptamer-based fluorescence polarization sensor dedicated to the small target analysis.

Sandrine Perrier¹, Corinne Ravelet, Valérie Guieu

¹Département de Pharmacochimie Moléculaire UMR 5063 CNRS, ICMG FR 2607, Université Grenoble I, Campus universitaire, 70, rue de la Chimie, 38240 Saint-Martin d'Hères, France.

Biosensors & Bioelectronics

|December 26, 2009

Summary

This study presents a new fluorescence polarization (FP) assay for small molecule sensing using engineered DNA aptamers. The method detects target binding by observing changes in fluorescence anisotropy, enabling sensitive detection and chiral analysis.

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Last Updated: Jun 17, 2026

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Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

Fluorescence polarization (FP) assays are valuable for detecting molecular interactions.
Nucleic acid aptamers offer specific molecular recognition capabilities.
Existing FP assays often require complex designs or lack versatility.

Purpose of the Study:

To develop a generalized fluorescence polarization (FP) sensor methodology.
To engineer aptamer instability for enhanced signal transduction.
To enable sensitive detection and chiral analysis of small molecules.

Main Methods:

Engineered an anti-adenosine DNA aptamer with a destabilized stem-loop structure.
Utilized a 3'-end fluorescein-labeled aptamer to monitor changes in dye mobility.
Applied the assay to detect adenosine and adenosine monophosphate (AMP).

Main Results:

A destabilized aptamer conformation increased local dye mobility and signal depolarization.
Target binding induced a stable aptamer structure, reducing dye mobility and increasing fluorescence anisotropy.
The assay successfully detected adenosine and AMP with high sensitivity.

Conclusions:

The rational design of aptamer instability provides a versatile FP sensing platform.
This method allows for sensitive detection and chiral discrimination of small molecules.
The approach holds promise for various applications in molecular sensing.