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Updated: Jun 17, 2026
Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
[Screening of proteins binding to FXR1P using yeast two-hybrid technique]
Jiao Su1, Shu-ya He, Bin-yuan Li
1Department of Biochemistry, Changzhi Medical College, Changzhi 046000, China. sujiao1028@126.com
Objective:
To screen the proteins interacting with FXR1P for functional investigation of FXR1P.
Methods:
The yeast strain AH109 transformed with the recombinant expression vector pGBKT7/FXR1 was mated with the yeast strain Y187 pretransformed with human fetal brain cDNA library. The positive clones were screened and identified by sequence analysis.
Results:
The recombinant expression vector pGBKT7/FXR1 was constructed successfully. Five proteins binding to FXR1P were screened from human fetal brain cDNA library using the yeast two-hybrid system, including CMAS, FTH1, GOLGA4, HSD17B1 and CSH1.
Conclusions:
These results provide new clues for investigating the biological functions of FXR1P and the pathogenesis of Fragile X syndrome.
