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Updated: Jun 17, 2026
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
Published on: August 26, 2020
Rapid detection of solute carrier family 4, member 1 (SLC4A1) mutations and polymorphisms by high-resolution melting
Choochai Nettuwakul1, Nunghathai Sawasdee, Pa-thai Yenchitsomanus
1Division of Medical Molecular Biology, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Objective:
The objective of this study is to develop and evaluate a high-resolution melting (HRM) method for detection of SLC4A1 mutations and polymorphisms.
Design And Methods:
The HRM method was optimized for detection of 18 known SLC4A1 variants. It was then used for analysis of 16 blind DNA samples highly enriched with two common mutations, Southeast Asian ovalocytosis (SAO) and band 3 Bangkok 1 (G701D), to compare the results with that of the conventional procedures.
Results:
The HRM method was able to detect all 18 SLC4A1 variants. In the samples in which homozygous wild-type and homozygous variant could not be distinguished by difference plots, they were spiked with a sample carrying known homozygous genotype, resulting in their clear differentiation. The HRM method had 100% efficiency for detection of mutations in the blind DNA samples, when compared with that of the conventional techniques.
Conclusions:
The developed HRM method is efficient and reproducible for detection of SLC4A1 mutations and polymorphisms.
