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Related Experiment Video

Updated: Jun 17, 2026

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
14:28

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

Published on: March 16, 2016

Analyzing neurite outgrowth from explants by fitting ellipses.

Carmen Haines1, Geoffrey J Goodhill

  • 1The Queensland Brain Institute, The University of Queensland, St Lucia 4072, Queensland, Australia.

Journal of Neuroscience Methods
|December 29, 2009
PubMed
Summary
This summary is machine-generated.

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This study introduces a new ellipse-fitting method to analyze nerve fiber growth patterns from neural explants. This technique reveals new insights into how molecular cues guide neurite development in the nervous system.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Nervous system development relies on molecular guidance cues for nerve fiber connectivity.
  • Neural explant culture in 3D collagen gels is a common method to study responses to these cues.
  • Previous analyses of neurite outgrowth patterns were limited in scope.

Purpose of the Study:

  • To develop a sophisticated method for characterizing neurite outgrowth patterns from neural explants.
  • To apply this new method to analyze dorsal root ganglion explants exposed to nerve growth factor gradients.
  • To gain new insights into neurite guidance by molecular cues.

Main Methods:

  • Developed an ellipse-fitting method to analyze neurite outgrowth patterns.
  • Characterized outgrowth using 5 parameters: ellipse center (x, y), elongation, area, and tilt.

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Last Updated: Jun 17, 2026

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
14:28

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

Published on: March 16, 2016

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons
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A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

Published on: August 6, 2020

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement
05:28

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement

Published on: August 9, 2024

  • Applied the method to a dataset of dorsal root ganglion explants cultured with nerve growth factor gradients.

    • Main Results:

    • The ellipse-fitting method provided a more detailed characterization of neurite outgrowth.
    • Analysis revealed that the ellipse's center position, not its shape, correlated with gradient strength.
    • New features of neurite guidance by molecular cues were identified.

    Conclusions:

    • Ellipse-fitting offers a powerful approach to quantitatively analyze neurite outgrowth from explants.
    • This method enhances understanding of the biological processes governing neurite guidance.
    • The findings highlight the importance of precise positional information in neural development.