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A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

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An in-situ fluorescence-based optical extensometry system for imaging mechanically loaded bone.

Christopher Price¹, Wen Li, John E Novotny

¹Center for Biomedical Engineering Research, Department of Mechanical Engineering, University of Delaware, Newark, Delaware 19716, USA.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society

|December 31, 2009

Summary

Researchers developed a novel optical extensometry system for confocal microscopy to measure tissue strain in small musculoskeletal samples. This tool enables detailed study of how mechanical forces influence cellular processes and tissue adaptation.

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Area of Science:

Musculoskeletal research
Biomedical engineering
Cellular mechanobiology

Background:

Accurate strain measurement is crucial for understanding musculoskeletal tissue adaptation.
Existing extensometry systems are often unsuitable for small biological samples.
Advanced imaging techniques require integrated loading and strain measurement tools.

Purpose of the Study:

To develop and validate a fluorescence-based optical extensometry system for confocal microscopy.
To enable in situ measurement of surface strain on small bone samples.
To facilitate the study of cellular processes under mechanical loading.

Main Methods:

Integration of a fluorescence-based optical extensometry system with a confocal microscopy platform.
Validation using well-characterized materials (bovine femoral cortex) and intact murine tibia.
Measurement of surface strains in the physiological range (200-3000 microstrain).

Main Results:

The developed system accurately and reproducibly measures surface strains in small bone samples.
The system is compatible with direct imaging of cellular processes.
Physiologically relevant strains were successfully quantified.

Conclusions:

This optical extensometry system provides a powerful tool for musculoskeletal research.
It enables investigation of the relationship between mechanical loading and mechanosensation.
Facilitates studies on fluid and solute transport in response to mechanical stimuli.