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Updated: Jun 17, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
Published on: February 23, 2016
L-band Overhauser dynamic nuclear polarization
Sandra Garcia1, Jeffrey H Walton, Brandon Armstrong
1Department of Food Science & Technology, University of California, Davis, CA 95616, USA. spgarcia24@gmail.com
Abstract:
We present the development of an Overhauser dynamic nuclear polarization (DNP) instrument at 0.04 T using 1.1 GHz (L-band) electron spin resonance frequencies (ESR) and 1.7 MHz (1)H nuclear magnetic resonance frequencies. Using this home-built DNP system, the electron-nucleus coupling factor of 4-oxo-TEMPO dissolved in water was determined as 0.39+/-0.06 at 0.04 T. The higher coupling factor obtained at this field compared to higher magnetic fields, such as 0.35 T, directly translates to higher enhancement of the NMR signal and opens up a wider time scale window for observing water dynamics interacting with macromolecular systems, including proteins, polymers or lipid vesicles. The higher enhancements obtained will facilitate the observation of water dynamics at correlation times up to 10 ns, that corresponds to more than one order of magnitude slower dynamics than accessible at 0.35 T using X-band ESR frequencies.
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