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Two-Step Purification of Cordycepin from Cordyceps Millitaris by High-Speed Countercurrent Chromatography
Xiuyun Ju1, Yong Sun, Xiaoying Cao
1Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China.
Journal of Liquid Chromatography & Related Technologies
|January 5, 2010
Abstract:
Cordycepin is successfully isolated and purified from Cordyceps millitaris in two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatography analysis.

