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A two-photon fluorescent probe for thiols in live cells and tissues.

Jun Han Lee¹, Chang Su Lim, Yu Shun Tian

¹Department of Chemistry, Korea University, 1-Anamdong, Seoul, 136-701, Korea.

Journal of the American Chemical Society

|January 8, 2010

Summary

We developed a new two-photon fluorescent probe for detecting thiols in live cells and tissues. This probe allows for deep tissue imaging without interference, advancing biological research.

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Area of Science:

Chemical Biology
Biomedical Imaging
Molecular Probes

Background:

Thiols are crucial biological molecules involved in various cellular processes.
Accurate detection of thiols in live biological systems is essential for understanding cellular functions.
Existing detection methods may face limitations in sensitivity, specificity, or depth penetration.

Purpose of the Study:

To develop and characterize a novel two-photon fluorescent probe for sensitive and selective thiol detection.
To demonstrate the probe's utility in live-cell and live-tissue imaging.
To enable deep-tissue imaging of thiols using two-photon microscopy.

Main Methods:

Synthesis and characterization of the two-photon fluorescent probe (ASS).
Evaluation of probe's photophysical properties and selectivity for thiols.
Application of the probe in two-photon microscopy for imaging thiols in live cells and tissues at depths of 90-180 microm.

Main Results:

The developed probe (ASS) exhibits efficient two-photon excitation at 780 nm.
The probe demonstrates high sensitivity and selectivity for detecting thiols.
Successful imaging of thiols in live cells and tissues at significant depths (90-180 microm) was achieved.
The probe showed no interference from other biologically relevant species during imaging.

Conclusions:

The novel two-photon fluorescent probe (ASS) is a powerful tool for thiol detection in biological samples.
The probe enables deep-tissue imaging of thiols with high specificity and minimal interference.
This technology has significant potential for advancing research in chemical biology and biomedical imaging.