Related Experiment Video
Updated: Jun 17, 2026

Culturing Primary Rat Inner Medullary Collecting Duct Cells
Published on: June 21, 2013
Carbonic anhydrase II is secreted from bovine parotid glands
M Mau1, T M Kaiser, K-H Südekum
1Institute of Animal Science, University of Bonn, Bonn, Germany. mmau@itw.uni-bonn.de
Abstract:
Besides carbonic anhydrase VI (CA-VI), CA-II is suggested to be a second secreted isoenzyme in ruminant saliva. Therefore, the aim of the present study was to investigate the expression of salivary CA-II in bovine parotid glands at the protein level. Moreover, we intended to identify the cells which secrete the enzyme into the saliva. Two commercially available CA-II specific antibodies were tested for use in immunohistochemistry on frozen sections of bovine parotid tissue. Intense positive staining for CA-II was found in luminal duct cells and for the first time also inside the duct lumen, clearly demonstrating the expression and secretion of salivary CA-II in bovine parotid glands. The presence of CA-II protein was verified for parotid tissue and whole saliva using immunoblot analysis. Both salivary CA-II and CA-VI are highly active in supplying the alimentary tract with bicarbonate. It is suggested that a decrease in the expression of either one of these enzymes might severely disturb digestion and/or increase susceptibility to acidosis in ruminants.
Related Concept Videos
Reabsorption and Secretion in the DCT and Collecting Duct
The distal part of the DCT, along with the...
The Parathyroid Glands
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by producing...
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Renal Regulation of Acid-Base Balance
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
The Pituitary Gland
Cranial Part of Parasympathetic Division
The vagus nerve (cranial nerve X) alone accounts for approximately 75...

