Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Electrodeposition

Electrodeposition

Electrodeposition is a technique used to separate an analyte from interferents by electrochemical processes. Here, the analyte is a metal ion that can be deposited on an electrode immersed in the sample solution. The electrochemical setup consists of an anode and a cathode. When an electric current is applied to the setup, oxidation occurs at the anode. At the cathode, which consists of a large metal surface, metal ions undergo reduction and deposit onto the surface.
Electrodeposition can...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

Electrogravimetric Analysis: Overview

Electrogravimetric Analysis: Overview

Electrogravimetric analysis measures the weight of an analyte deposited electrolytically onto a suitable working electrode. This method involves applying a potential to a pre-weighed electrode submerged in a solution, which results in the desired substance being deposited through reduction at the cathode or oxidation at the anode. The electrode's weight is recorded after deposition, and the difference in weight gives the analyte's weight in the solution.
To test the completeness of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synthetic Multiepitope Protein-Based Plasmonic Immunosensor for Rapid, Label-Free Detection of <i>Cryptococcus</i> Antibodies in Human Serum.

ACS infectious diseases·2025

Same author

Strategies for Electrochemical Point-of-Care Biosensors.

Annual review of analytical chemistry (Palo Alto, Calif.)·2025

Same author

Introducing all-inkjet-printed microneedles for in-vivo biosensing.

Scientific reports·2024

Same author

Photoelectrochemical determination of phloroglucinol based on a combination of a ceramic perovskite and bismuth vanadate.

Mikrochimica acta·2024

Same author

CRISPR-based electrochemical biosensors: an alternative for point-of-care diagnostics?

Talanta·2024

Same author

Advanced Hybrid materials in electrochemical sensors: Combining MOFs and conducting polymers for environmental monitoring.

Chemosphere·2024

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors

Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors

Published on: November 22, 2016

Electrochemical detection in a paper-based separation device.

Rafaela Fernanda Carvalhal¹, Marta Simão Kfouri, Maria Helena de Oliveira Piazetta

¹Analytical Chemistry Department, Institute of Chemistry State University of Campinas-UNICAMP, SP, P.O. Box 6154, Campinas, Brazil.

Analytical Chemistry

|January 9, 2010

Summary

Researchers developed novel microfluidic paper-based devices for simple, low-cost chemical analysis. These devices successfully separated and quantified uric and ascorbic acid, offering a new analytical method.

More Related Videos

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Bacterial Detection & Identification Using Electrochemical Sensors

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Related Experiment Videos

Last Updated: Jun 17, 2026

Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors

Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors

Published on: November 22, 2016

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Bacterial Detection & Identification Using Electrochemical Sensors

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Area of Science:

Analytical Chemistry
Microfluidics
Electrochemistry

Background:

Microfluidic devices offer miniaturized platforms for chemical analysis.
Paper-based devices provide a low-cost and accessible alternative to traditional laboratory equipment.
Electrochemical detection is a sensitive method for quantifying various analytes.

Purpose of the Study:

To develop and evaluate microfluidic paper-based separation devices with amperometric detection.
To demonstrate the capability of these devices for separating and quantifying specific compounds.

Main Methods:

Fabrication of a gold electrochemical microcell on polyester using photolithography.
Coupling of the microcell to a paper strip for chromatographic separation.
Amperometric detection for quantification of analytes.

Main Results:

Successful separation and quantification of uric acid and ascorbic acid in mixtures.
Demonstration of the device's analytical performance.
Validation of the integrated microfluidic paper-based system.

Conclusions:

The developed microfluidic paper-based devices offer a viable analytical alternative.
The method is suitable for situations prioritizing low cost and simplicity.
This technology has potential for point-of-care or field applications.