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Updated: Jun 17, 2026

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
Published on: July 10, 2016
Effect of a negatively charged lipid on membrane-lacticin Q interaction and resulting pore formation
Fuminori Yoneyama1, Kouki Shioya, Takeshi Zendo
1Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
Abstract:
Lacticin Q is an antimicrobial peptide that forms pores on membranes. We investigated effects of negatively charged lipids on the binding and pore formation of lacticin Q with liposomes by surface plasmon resonance analysis and fluorescence dye leakage experiments respectively. Negatively charged lipids accelerated the binding of lacticin Q on the membranes and the resulting pore formation. However, the acceleration was not an essential factor in the killing activity of lacticin Q, since pore-forming activities against electrically neutral and negatively charged liposomes occurred similarly.
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