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Glycopeptides from epithelial cell mutants: temperature sensitive for the transformation phenotype
International Journal of Cancer
|November 15, 1977
Summary
Cell surface glycopeptide profiles differ between normal and transformed epithelial cells. A temperature-sensitive mutant shows reversible changes in glycopeptide elution based on growth temperature, indicating altered cell surface characteristics.
Area of Science:
- Cell Biology
- Biochemistry
- Glycobiology
Background:
- Cell surface glycoproteins play crucial roles in cellular processes.
- Alterations in cell surface glycosylation are hallmarks of cellular transformation and cancer.
Purpose of the Study:
- To compare fucose-labelled glycopeptides from normal and transformed epithelial cells.
- To investigate the effect of temperature on glycopeptide profiles of a temperature-sensitive transformed cell line.
Main Methods:
- Co-chromatography using Sephadex G-50 gel filtration.
- Labelling of cell surface glycopeptides with fucose.
- Culturing of normal, transformed, and temperature-sensitive epithelial cells at different temperatures.
Main Results:
- Transformed and hepatoma cell glycopeptides eluted earlier than normal cell glycopeptides.
- A temperature-sensitive mutant (TS 223) exhibited altered glycopeptide profiles at non-permissive temperatures (40°C) compared to permissive temperatures (36°C).
- Differences were more pronounced in confluent cultures and reversible upon temperature shifts, with a lag period of at least 6 hours.
Conclusions:
- Cellular transformation is associated with distinct changes in cell surface fucose-containing glycopeptides.
- Temperature-sensitive alterations in glycopeptide profiles reflect changes in the transformed phenotype.
- These findings highlight the dynamic nature of cell surface glycosylation in response to environmental cues and transformation status.