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Related Concept Videos

Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

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Related Experiment Video

Updated: Jun 17, 2026

Pull-down of Calmodulin-binding Proteins
07:51

Pull-down of Calmodulin-binding Proteins

Published on: January 23, 2012

Calmodulin-binding proteome in the brain.

Zhiqun Zhang1, Firas H Kobeissy, Andrew K Ottens

  • 1Department of Psychiatry, University of Florida, Gainesville, FL, USA. zqzhang@banyanbio.com

Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2010
PubMed
Summary
This summary is machine-generated.

Researchers identified 69 potential calmodulin-binding proteins (CaMBPs) in the rat brain using a novel Ca(2+) sensor method. This technique helps map the brain

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Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
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Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

Related Experiment Videos

Last Updated: Jun 17, 2026

Pull-down of Calmodulin-binding Proteins
07:51

Pull-down of Calmodulin-binding Proteins

Published on: January 23, 2012

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

Area of Science:

  • Neuroscience
  • Proteomics
  • Biochemistry

Background:

  • Calcium dyshomeostasis is implicated in various neuropathological conditions, including traumatic brain injury (TBI), stroke, and neurodegenerative diseases.
  • Calmodulin (CaM), a crucial calcium (Ca2+) sensor in the brain, regulates essential signaling pathways by binding to downstream calmodulin-binding proteins (CaMBPs).

Purpose of the Study:

  • To develop and validate a CaM-affinity capture method coupled with RPLC-MSMS for studying the calcium-dependent CaM-binding proteome in the rat brain.
  • To identify known and novel CaMBPs involved in calcium signaling in the brain.

Main Methods:

  • Developed a CaM-affinity capture technique.
  • Utilized reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MSMS) for protein identification.
  • Applied the method to analyze the rat brain proteome.

Main Results:

  • Identified a total of 69 potential CaMBPs in the rat brain.
  • Confirmed 26 known CaMBPs and discovered 43 putative novel CaMBPs.
  • Demonstrated the efficacy of the CaM-affinity capture RPLC-MSMS approach.

Conclusions:

  • The CaM-affinity capture coupled with tandem mass spectrometry is an effective tool for constructing a brain CaM-binding proteomic network.
  • This methodology can be adapted to investigate alterations in the calmodulin-binding proteome in neurological, neurodegenerative, and psychiatric disorders.