Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developmental expression of Sp1 in the mouse.

J D Saffer1, S P Jackson, M B Annarella

  • 1Jackson Laboratory, Bar Harbor, Maine 04609.

Molecular and Cellular Biology
|April 1, 1991
PubMed
Summary

The transcription factor Sp1 (Specificity Protein 1) shows significant expression differences across mouse cell types and developmental stages. Elevated Sp1 levels correlate with cellular differentiation, suggesting a key regulatory role.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic determinants of micronucleus formation in vivo.

Nature·2024
Same author

Valproic acid selectively increases vascular endothelial tissue-type plasminogen activator production and reduces thrombus formation in the mouse.

Journal of thrombosis and haemostasis : JTH·2016
Same author

Survivability of Escherichia coli O157:H7 in mechanically tenderized beef steaks subjected to lactic acid application and cooking under simulated industry conditions.

Journal of food protection·2013
Same author

Differentiating haemostasis from thrombosis for therapeutic benefit.

Thrombosis and haemostasis·2013
Same author

Storage length, storage temperature, and lean formulation influence the shelf-life and stability of traditionally packaged ground beef.

Meat science·2013
Same author

The heritabilities, phenotypic correlations, and genetic correlations of lean color and palatability measures from longissimus muscle in beef cattle.

Journal of animal science·2013

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Specificity Protein 1 (Sp1) is a crucial transcription factor involved in regulating gene expression.
  • Its ubiquitous expression suggests a general role in cellular functions, including housekeeping gene transcription.

Purpose of the Study:

  • To investigate the expression patterns of the Sp1 transcription factor in different cell types and developmental stages in mice.
  • To determine if Sp1 expression levels correlate with cellular differentiation processes.

Main Methods:

  • Utilized RNA analysis to quantify Sp1 messenger RNA (mRNA) levels.
  • Employed immunohistochemical localization to determine Sp1 protein expression and distribution.

Main Results:

  • Sp1 mRNA and protein levels exhibited significant, unexpected variations (over 100-fold) across different cell types.
  • Expression levels of Sp1 varied notably during different developmental stages.
  • Highest Sp1 levels were observed in developing hematopoietic cells, fetal cells, and spermatids.

Conclusions:

  • Sp1 expression is dynamically regulated and not uniform across all cell types or developmental time points.
  • The observed correlation between elevated Sp1 levels and differentiation suggests a specific regulatory function beyond housekeeping genes.
  • These findings highlight Sp1's multifaceted role in cellular processes and development.

Related Experiment Videos