Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A workbench for multiple alignment construction and analysis.

G D Schuler1, S F Altschul, D J Lipman

  • 1National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894.

Proteins
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequence alignment and database searching.

Methods of biochemical analysis·2001
Same author

Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements.

Nucleic acids research·2001
Same author

Molecular profiling of clinical tissues specimens: feasibility and applications.

The Journal of molecular diagnostics : JMD·2001
Same author

Guide to the draft human genome.

Nature·2001
Same author

The estimation of statistical parameters for local alignment score distributions.

Nucleic acids research·2001
Same author

Lineage-specific loss and divergence of functionally linked genes in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2000
Same journal

Engineered HSP90-MP65 Bivalent Fusion Antigen: A Novel Vaccine Candidate Against Invasive Candidiasis.

Proteins·2026
Same journal

Physics-Based Energy Functions for Computational Protein Design.

Proteins·2026
Same journal

Impact of Stabilizing Osmolytes on the Conformational Dynamics of Human and Rat Islet Amyloid Polypeptides.

Proteins·2026
Same journal

Stabilization of Bone Morphogenetic Protein-2 at Physiological pH: Contrasting Roles of CHAPS and Arginine in Aggregation Inhibition.

Proteins·2026
Same journal

Structural Insights Into the Function of Leishmania major Adenylosuccinate Lyase.

Proteins·2026
Same journal

Generalizing the Gaussian Network Model: Spanning-Tree Thermodynamics Shows Entropy-Driven KRAS Activation.

Proteins·2026
See all related articles

MACAW is a new interactive program for multiple sequence alignment, aiding molecular evolution studies. It offers novel features for block searching, statistical evaluation, and flexible editing to tailor alignment strategies.

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Molecular Evolution

Background:

  • Multiple sequence alignment is crucial for understanding molecular evolution and sequence-structure-function relationships.
  • Existing methods for multiple sequence alignment have limitations in handling complex biological data.

Purpose of the Study:

  • To develop an interactive program, MACAW (Multiple Alignment Construction and Analysis Workbench), for constructing and analyzing multiple sequence alignments.
  • To introduce novel features that improve the accuracy and flexibility of multiple sequence alignment.

Main Methods:

  • Developed a new search algorithm for locating regions of local similarity.
  • Incorporated a recently developed mathematical theory for evaluating the statistical significance of similarity blocks.

Related Experiment Videos

  • Implemented a user interface with visualization tools for block evaluation and editing.

    • Main Results:

    • MACAW allows users to construct multiple alignments by locating, analyzing, editing, and combining aligned sequence segments (blocks).
    • The program features a novel similarity search algorithm and statistical evaluation of similarity blocks.
    • Flexible editing tools enable users to refine alignments by adjusting block boundaries and segments.

    Conclusions:

    • MACAW provides a powerful and flexible tool for multiple sequence alignment, suitable for diverse research needs.
    • The interactive approach, combining advanced algorithms with user control, addresses the complexities of multiple alignment construction.