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Updated: Jun 17, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
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Computer processing of spectrograms for absolute intensities.

A Guttman1, J Golden, H J Galbraith

  • 1General Electric Company, King of Prussia, Pennsylvania, USA.

Applied Optics
|January 12, 2010
PubMed
Summary
This summary is machine-generated.

A new computer program accurately processes photographic spectra for absolute intensity. This method achieves radiometric accuracy within 10% for spectral analysis using common photographic emulsions.

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Area of Science:

  • Photographic science
  • Spectroscopy
  • Image processing

Background:

  • Accurate intensity measurements are crucial for spectral analysis.
  • Traditional methods for processing photographic spectra can be labor-intensive and prone to error.
  • Developing automated, precise methods is essential for advancing spectroscopic research.

Purpose of the Study:

  • To develop and validate a computer program for processing photographically recorded spectra.
  • To achieve accurate absolute intensity measurements from densitometric scans.
  • To evaluate the performance of the program with common photographic emulsions.

Main Methods:

  • Densitometric scanning of test and calibration films to obtain digital density data.
  • Fitting nonlinear calibration data using least-squares cubic polynomials to approximate H&D curves.
  • Applying a sensitivity threshold adjustment to enhance radiometric accuracy.

Main Results:

  • The developed program processes absolute intensities with an accuracy of within 15%.
  • Adjusting the sensitivity threshold improved radiometric accuracy to within 10%.
  • Generated gamma vs wavelength curves revealed potential evidence of the photographic Purkinje effect in 2475 emulsion.

Conclusions:

  • The computer program provides a reliable method for absolute intensity processing of photographic spectra.
  • The findings demonstrate the effectiveness of cubic polynomial fitting and sensitivity threshold adjustment for improving accuracy.
  • Further investigation into the photographic Purkinje effect in specific emulsions is warranted.