Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial Mapping of Electrochemical Hole Injection into Supercrystals of Perovskite Nanocrystals.

Nano letters·2026
Same author

Robust Hybrid Plasmon-Photon Modes in Colloidal Metasurfaces Probed by Angle-Resolved SERS.

ACS applied materials & interfaces·2026
Same author

Recycling Ag SERS-substrates from strongly chemisorbing molecules.

Nanoscale advances·2026
Same author

The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

TBHubbard: tight-binding and extended Hubbard model dataset for metal-organic frameworks.

Scientific data·2025
Same author

Mechanisms and reversibility of glyphosate and phosphorus ligands sorption on Al<sub>2</sub>O<sub>3</sub>: Experimental evidence and computational modeling.

Water research·2025

Related Experiment Video

Updated: Jun 17, 2026

Fabrication and Testing of Microfluidic Optomechanical Oscillators
09:10

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Published on: May 29, 2014

Three-dimensional orientation of single molecules in a tunable optical lambda/2 microresonator.

Raphael Gutbrod1, Dmitry Khoptyar, Mathias Steiner

  • 1Institute of Physical and Theoretical Chemistry, Eberhard-Karls-University Tuebingen, 72076 Tuebingen, Germany.

Nano Letters
|January 13, 2010
PubMed
Summary

Researchers used single molecules to map laser light patterns in a microresonator. This technique precisely determined the molecule

More Related Videos

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators
12:21

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators

Published on: April 4, 2016

Related Experiment Videos

Last Updated: Jun 17, 2026

Fabrication and Testing of Microfluidic Optomechanical Oscillators
09:10

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Published on: May 29, 2014

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators
12:21

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators

Published on: April 4, 2016

Area of Science:

  • Optics and Photonics
  • Molecular Biophysics
  • Nanotechnology

Background:

  • Optical microresonators confine light, enabling enhanced light-matter interactions.
  • Radially polarized laser beams exhibit unique field distributions.
  • Understanding vector properties of light fields is crucial for nanoscale applications.

Purpose of the Study:

  • To investigate the bimodal field distribution of a radially polarized laser beam within an optical lambda/2-microresonator.
  • To utilize single-molecule dipoles as probes for vector field properties.
  • To determine the three-dimensional orientation of a single-molecule dipole.

Main Methods:

  • Employing a tightly focused radially polarized laser beam.
  • Utilizing a single-molecule dipole as a nanoscale sensor.
  • Precisely tuning the microresonator length with nanometer accuracy.
  • Comparing experimental excitation patterns with theoretical calculations.

Main Results:

  • Observed an unusual bimodal field distribution generated by the laser beam in the microresonator.
  • Successfully probed the vector properties of the light field using the single-molecule dipole.
  • Achieved nanometer-precision control over the resonator length to map field variations.
  • Experimental data matched calculated excitation patterns, validating the approach.

Conclusions:

  • The study demonstrates a novel method for characterizing complex optical fields at the nanoscale.
  • Single-molecule probing provides a sensitive tool for mapping vector field distributions.
  • This technique enables precise determination of single-molecule dipole orientation within optical microcavities.